Near‐Infrared Lifetime Imaging of Biomolecules with Carbon Nanotubes
نویسندگان
چکیده
Einwandige Kohlenstoffnanoröhren (single-walled carbon nanotubes, SWCNTs) sind vielseitig einsetzbare Bausteine für Biosensoren, die im nahen Infrarot (NIR) fluoreszieren. Ihre Oberfläche kann chemisch so modifiziert werden, dass sie auf Analyten mit einer Veränderung ihrer Fluoreszenz reagieren. Intensitätsbasierte Signale werden jedoch leicht durch äußere Faktoren wie Bewegungen der Probe beeinflusst. Hier zeigen wir Fluoreszenz-Lebensdauer Mikroskopie (fluorescence lifetime imaging microscopy, FLIM) von SWCNT-basierten Sensoren NIR. Dafür wurde ein konfokales Laser-Scanning-Mikroskop (CLSM) NIR-Signale (>800 nm) angepasst und zeitkorrelierte Einzelphotonenzählung (GT)10-DNA-funktionalisierten SWCNTs verwendet. (GT)10-SWCNTs fungieren als den wichtigen Neurotransmitter Dopamin. Fluoreszenzlebensdauer (>900 fällt biexponentiell ab, wobei längere Lebensdauerkomponente (370 ps) steigender Dopaminkonzentration um bis zu 25 % ansteigt. Mit diesen können Zellen beschichtet extrazelluläres Dopamin in 3D mittels FLIM messen. Wir demonstrieren damit das Potenzial Messgröße SWCNT-basierte NIR-Sensoren.
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ژورنال
عنوان ژورنال: Angewandte Chemie
سال: 2023
ISSN: ['1521-3773', '1433-7851', '0570-0833']
DOI: https://doi.org/10.1002/ange.202300682